Cisplatin gsh

Author: ekki

August undefined, 2024

WebJul 29, 2024 · Glutathione (GSH) is the most abundant non-protein thiol in cells and plays a critical role in maintaining intracellular redox homeostasis. 1 Alterations in glutathione levels are associated with several cellular … Webized GSH concentration as compared to cisplatin alone (table 2). The renal GSH and oxidized GSH concentrations in lipoic acid alone treated group were 19.93”2.39 and 1.14”0.12 nmoles/mg protein which were close to control values. Superoxide dismutase activity in the kidney signiﬁcantly decreased (62% of control) in cisplatin-treated rats ...

A GSH-depleted platinum(IV) prodrug triggers ... - ScienceDirect

Web(GSH) transferase activity of GST P1-1. Instead, GST P1-1 sequesters and inactivates cisplatin with the aid of 2 solvent-accessible cysteines, resulting in protein subunits cross-linking, while maintaining its GSH-conjugation activity. Furthermore, it is well known that GST P1-1 binding to the c-Jun N-terminal kinase (JNK) inhibits JNK phosphory- WebOct 1, 2024 · In brief, the reduction of GSH can rescue the inactivation of cisplatin, increase sensitivity of cancer cells to platinum drugs. As shown in Fig. S1A (Supporting information), CDDP-OH was firstly synthesized through oxidizing cisplatin by H 2 O 2 using the method previously reported [21]. popular now on him

A structure-based mechanism of cisplatin resistance …

WebDec 15, 2015 · HCT116 colon carcinoma was selected as cancer cell prototypes that overexpress GSH. 49 Cells were incubated for 6 hours with CDDP-containing samples (or controls) in order to mimic the clinically used intravenous (iv) infusion of cisplatin, and then further incubated for 66 hours in fresh media to assess the cytotoxicity profiles over a … Webized GSH concentration as compared to cisplatin alone (table 2). The renal GSH and oxidized GSH concentrations in lipoic acid alone treated group were 19.93”2.39 and … WebNov 1, 2015 · Increased GSH level has been associated with cisplatin resistance [11], [12], [13]. Cystine, the rate-limiting amino acid for GSH synthesis, is transported by the xc − system composed of heavy subunit F42hc, a common component for amino acid transport and the active subunit xCT [21]. shark protection forum

A ratiometric fluorescent probe for real-time …

Applied Sciences Free Full-Text Ginkgo biloba Alleviates Cisplatin ...

WebResults: Serum GSH content depleted significantly after a total dose of 4 Gy and 10 Gy of radiotherapy with a single dose of cisplatin, which was significantly lesser in NR than of CR patients. Similarly, Olive Tail Moment, the index of DNA damage, indicated significantly higher values in the fifth fraction of radiotherapy (5-RT) than in ... WebNeurotoxicity is an obvious adverse effect in Patients encountering a complete course of chemotherapy. The present work is conducted to evaluate the neuroprotective effect of Ginkgo biloba (Ginkgo) against the neurotoxicity induced by Cisplatin (Cis) in rats. Forty male Wistar albino rats were arranged into four groups: (1) Control group, rats were … popular now on h disappearedWebGSH reacts with hydrogen peroxide and organic peroxides, the harmful byproducts of aerobic life. GSH is also essential for maintaining the normal structure of red blood cells.4 In the presence of cisplatin, GSH forms a 2:1 (GSH:platinum) complex … popular now on home disappear

"WebMar 13, 2006 · Cisplatin is one of the most widely used chemotherapeutic drugs for treating cancer. Initially, platinum-based therapies are very effective in treating a wide array of cancers; however, recurrence and … " - Cisplatin gsh

Cisplatin gsh

A structure-based mechanism of cisplatin resistance …

WebAug 8, 2010 · Cisplatin can bind to the thiol group of intracellular glutathione (GSH) and this reaction is considered important for the inactivation of cisplatin . However, Gibson and coworkers recently suggested that binding of cisplatin to GSH is not the most important cellular interaction for inactivation ( 17 ). WebCisplatin is a platinum-based agent whose nephrotoxicity is thought related to the chloride in the cis position. Cisplatin gains entry into tubular cells via uptake by the OCT-2 …

Did you know?

WebMar 20, 2024 · GSH-depleting agents buthionine sulfoximine is a useful agent for inhibition of γ-glutamylcysteine ligase, and it reduced the resistance to cisplatin in malignant glioma (Rocha et al., 2015). However, non-specific GSH depletion can cause irreversible damage in most normal tissues. WebJul 1, 2013 · GSH concentration was measured with UV-Visible double beam spectrophotometer, using the Ellman’s method. The level of GSH was lowered upon addition of cisplatin to plasma and cytosolic...

WebJun 20, 2024 · We propose here a new mechanism of cisplatin resistance mediated by glutathione transferase (GST) P1-1, as a cisplatin-binding protein. Our results show that … WebFeb 1, 2012 · Recently, Volckova, et al. [17] used a variety of physical techniques to show that GSH rapidly reduces iproplatin, 2, to produce cis -di (isopropylamine)chloro-glutathionatoplatinum (II) which has the thiolate of a GSH molecule bound to Pt (II).

WebFeb 28, 2024 · Three mechanisms define cisplatin’s direct involvement, demonstrating the chemo-resistant tumor cell phenotype: pre-target, on-target, and post-target resistance [20,21,22]. The first is characterized by reduced intracellular accumulation and increased cisplatin sequestration caused by GSH and other scavengers. WebAlthough cisplatin (cisPt) is one of the most often used cytotoxic drugs in the treatment of cancer, its clinical application is associated with nephrotoxicity 掌桥科研一站式科研服务平台

WebNov 27, 2024 · It is well established that one of the main factors that promote intracellular cisplatin detoxification is GSH, since it can bind covalently to the drug and prevent it to …

WebEbselen is a seleno-organic compound with documented cytoprotective properties. Little work has been done, however, demonstrating ebselen's cytoprotective prop popular now on hhjhWebThree mechanisms have been proposed for the role of glutathione (GSH) in regulating cisplatin (CDDP) sensitivities that affects its ultimate cell-killing ability: (i) GSH may … popular now on hghgWebThe therapeutic efficacy of cisplatin has been restricted by drug resistance of cancers. Intracellular glutathione (GSH) detoxification of cisplatin under the catalysis of … popular now on hlnWebSep 15, 2024 · Cisplatin Injection (cisplatin injection (cisplatin (cisplatin injection) injection) ) is a sterile aqueous solution, available in 50, 100 and 200 mL multiple dose … popular now on heageWebFeb 17, 2024 · The results showed that levels of glutamine, glutamate, and glutathione (GSH), a key drug resistance mediator synthesized from glutamate, were significantly elevated in A2780cis cells than those in A2780 cells. Furthermore, glutamine starvation decreased the GSH levels and CDDP resistance in A2780cis cells. shark protectionWebApart from GSH-conjugates, cisplatin can also be detoxified by binding to metallothioneins. Nevertheless, J82 did not show any difference in the amount of Pt-adducts between parental and LTT cells despite increased MT1B levels. However, we found metallothioneins mainly overexpressed in RT-112-LTT, suggesting that these cells evade cisplatin ... shark protection deviceWebPubMed Central (PMC) popular now on hl