WebMay 24, 2024 · Lysates from BT-549/CshRNA and BT-549/MUC1shRNA#2 were immunoblotted with antibodies against the indicated proteins (F, right). (G,H). BT-549/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days and stimulated with poly I:C for 12 h (G) or IFN-β for 4 h (H) were analyzed for RIG-I and MDA5 mRNA levels by qRT-PCR. … WebVector-based short hairpin RNA (shRNA) is a type of RNA interference (RNAi) technology leveraged to study the function of unknown genes. RNAi works by by silencing gene …
MUC1-C induces DNA methyltransferase 1 and represses …
WebJul 25, 2009 · RNA interference (RNAi) is a natural process through which expression of a targeted gene can be knocked down with high specificity and selectivity. Using available … WebShort hairpin RNA. Lentiviral delivery of shRNA and the mechanism of RNA interference in mammalian cells. A short hairpin RNA or small hairpin RNA ( shRNA /Hairpin Vector) is … portland to boston bus schedule
AFP promoter/enhancer +2 c-My cshRNA delivered by F-virosomes ...
WebJul 26, 2024 · cshRNA target prediction algorithm. A web based graphical user interface for the identification of target sites and generation of complete cshRNA designs is available … WebMDA-MB-468/CshRNA or MDA-MB-468/MUC1shRNA cells (data not shown). Treatment with the MUC1-C inhibitor, GO-203, was similarly associated with down-regulation of MEK and ERK phosphorylation (Fig. 1B). In addition, GO-203 treat-ment resulted in a 55 2% decrease in p-C/EBP levels (Fig. 1B, legend). In HER2-overexpressing SKBR3 breast … WebJun 21, 2024 · We also studied MDA-MB-468 triple-negative breast cancer (TNBC) cells expressing a control shRNA (CshRNA) or a MUC1-targeting shRNA in order to knock down MUC1-C expression . We found that binding of mAb 3D1 to MDA-MB-468/MUC1 shRNA cells was substantially decreased compared with that in MDA-MB-468/CshRNA cells ( … option analysis software india