Ctcf western
WebMar 3, 2024 · Western Blotting. Cells were lysed in buffer containing 1% (vol/vol) protease inhibitor cocktail (Sigma P2714) ... ChIP-seq identifies sites of BRD8 and CTCF occupancy genome-wide. A, B, The top 7 enriched motifs under BRD8 (A) or CTCF (B) ChIP-seq peaks in Calu-3 cells. Motifs are ranked by their % incidence in target and background regions. WebJun 5, 2024 · CTCF shRNA and controls were analyzed by western blotting using anti-CTCF rabbit monoclonal antibody (Cell Signaling #3418) to verify knockdown. Cell counting assays were performed using Hoest staining as previously described to measure response to CTCF knockdown. For 5-aza-2′-deoxycytidine treated plates, 0.2 uM of 5-aza-2 ...
Ctcf western
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WebJun 22, 2024 · CTCF is a key organizer of the 3D genome. Its specialized paralog, BORIS, heterodimerizes with CTCF but is expressed only in male germ cells and in cancer … WebAug 23, 2024 · CTCF and the cohesin complex are essential for stabilizing chromatin organization, which has divergent effects on gene regulation, embryonic development, …
WebProduct Information. 10915-1-AP targets CTCF in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. … WebBackground Information. CTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers.
WebAntibody specificity was demonstrated by siRNA mediated knockdown of target protein. U-251 mg cells were transfected with, control siRNA, CTCF siRNA probe #1, and CTCF siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using anti-CTCF Monoclonal Antibody (CL0304) (Product # MA5-31343) used at a dilution of 1 ... WebCTCF (CCCTC-binding factor, zinc finger protein) is a chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction …
WebThe purpose of the present study was to explore further the mechanism of CTCF controlling Pax6 gene expression in human retinoblastoma (Rb) cells and in the development of …
WebDec 23, 2008 · Western blot analysis demonstrated decreased CTCF protein in cells treated with the CTCF siRNA compared with control siRNA-treated cells (Fig. 6A). … smart and simple napkinsWebKnockdown of CTCF was achieved by transfecting MCF7 with CTCF specific siRNAs (Silencer® select Product # s20966, s20967). Western blot analysis (Fig. a) was performed using nuclear enriched extracts from the CTCF knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). smart and simple swf playerWebFeb 1, 2024 · CCCTC-binding factor (CTCF), a novel transcriptional repressor, was reported to participate in the occurrence of various cardiovascular diseases. The present … hill country bridgeWebDec 4, 2024 · CTCF western blot. To confirm the CTCF or RAD21 antibody, 0.5 million HEK 293 cells (obtained from ATCC: ATCC CRL-1573; tested for mycoplasma contamination but not authenticated) and 0.5 million ... smart and skilled application loginWebTranscriptional factor CTCF is a ubiquitously expressed 11-zinc finger domain containing, DNA-binding nuclear phosphoprotein that is involved … smart and simple hotels tunbridge wellsWebApr 11, 2024 · Pathological remodeling of atrial tissue renders the atria more prone to arrhythmia upon arrival of electrical triggers. Activation of the renin-angiotensin system is an important factor that contributes to atrial remodeling, which may result in atrial hypertrophy and prolongation of P-wave duration. In addition, atrial cardiomyocytes are electrically … hill country buck beddingWebCTCF is a nuclear protein initially discovered for its role in enhancer-promoter insulation. It has been shown to play a role in genome architecture and in fact, its DNA binding sites … hill country brewery tours