WebThermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and … WebAug 20, 1992 · The structure of bovine pancreatic deoxyribonuclease I (DNase I) has been refined at 2 A resolution using the restrained parameter, reciprocal least-squares procedure of Hendrickson and Konnert. The conventional R-factor for 16,104 reflections with I greater than or equal to 3 sigma (I) from 6.0 to 2.0 A resolution is 0.157.

Thermo Scientific™ DNase I, RNase-free (supplied with MnCl2) …

WebDNase Ito 20 A260 unit of nuclei) then incubated at 22†Ž for 15 min in 10 % sucrose, A bbreviations: TKM buffer, 50 mM Tris-HCl, pH 7.5-25 mM KCl -5 mM MgCl2; TM buffer, 50 mM Tris-HCl, pH 7.4-5 mM MgCl2; NMx DN, nuclear matrix prepared by DNase I digestion method; NMx EN, nuclear matrix prepared by ... WebDescription: Deoxyribonuclease I (DNase I) from beef pancreas is an endonuclease (glycoprotein), which preferentially cleaves the phosphodiester bond in the DNA behind pyrimidine nucleotides. This results in a polynucleotide with a 5'-phosphate and a free OH-group in position 3'. DNase I cleaves single-stranded and double-stranded DNA as well ... lauri pohjanpää

Thermo Scientific Reaction Buffer with MgCl2 for DNase I (10X ...

WebRequest bulk or custom quote. Thermo Scientific 10X Reaction Buffer with MgCl 2 is used with RNase-free DNase I, an endonuclease that digests single- and double-stranded … WebTumor tissues were harvested and digested with 2 mL of 1640 medium containing 5% FBS, 2 mg/mL collagenase IV (Sigma), and 5 U/mL DNase I (Sigma) for 20 min in a 37 °C incubator. Single cell suspensions were obtained after lysing the tissues with 2 mL of blood cell lysis buffer for 2 min and filtering through a 70 μm mesh. WebDec 17, 2014 · Deoxyribonucleases (DNases) are a class of enzymes able to catalyze DNA hydrolysis. DNases play important roles in cell function, while DNase inhibitors control or … lauri putkivaara