WebTo remove strongly retained materials from the column, flush the column in the reverse direction with very strong solvents such as 50:50 methanol:water. Mobile phase A mobile phase buffer pH that is close to …
Luna HPLC/UHPLC Columns Tips for Care and Use - Microsoft
Web1 day ago · Retention times with this column were highly stable provided a sufficient cleaning with high ionic strength solvents/chelators and conditioning with at least 10 column volumes of mobile phase A prior to actual measurements ... Therefore, we developed our final method using the BEH Z-HILIC column, the second best in terms of … WebJul 29, 2006 · HILIC Column Cleanup. We've been developing a HILIC method for analyzing amino acids in plasma. It worked fine but the peak shape degraded and retention time shifted rapidly after injecting samples prepared by protein crash. I suspect that the phase is bound with phospholipids, or perhaps the enzyme inhibitor added to the plasma. chumbera hongos
HILIC Troubleshooting Thermo Fisher Scientific - US
WebFlush column with 100% organic mobile phase (methanol or acetonitrile) by setting the pump flow rate to 0.1 mL/min. and increase the flow rate to 1 mL/min over 5 minutes. 3. When the mobile phase is flowing freely from the column outlet, stop the flow and attach the column outlet to the detector. WebHydrophilic Interaction Chromatography (HILIC) Columns (for LC/MS) Multi-Mode Columns (for LC/MS) Organic SEC (GPC) Columns (for LC/MS) Reversed Phase Columns (for LC/MS) ... GPC Clean-Up of Pesticides Residuals in Foods . GPC Clean-Up of Phthalic Acid Esters . N-Methylcarbamates . Other Pesticides . Proteins, Peptides and Amino Acids ... WebDec 20, 2024 · ANSWER. For a system with a fixed loop (examples: ACQUITY UPLC Binary, ACQUITY UPLC I-Class FL): Strong needle wash: 10:90 acetonitrile/water. Weak needle wash: 90:10 acetonitrile/water or the same as initial mobile phase (no buffer or additive) For a system with a flow-through needle (examples: ACQUITY UPLC H-Class, ACQUITY UPLC H … chum beverage